MBD0015

4′,6-Diamidino-2-phénylindole dihydrochloride

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

• Synonyme(s) : 2-(4-Amidinophényl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• Formule empirique (notation de Hill): C₁₆H₁₅N₅ · 2HCl
• Numéro CAS: 28718-90-3
• Poids moléculaire : 350.25
• Numéro Beilstein : 4894417
• Numéro MDL: MFCD00012681
• Code UNSPSC : 41105500

Propriétés

• Niveau de qualité: 200
• Forme: liquid
• Conditions de stockage: protect from light
• Concentration: 1 mg/mL
• Technique(s): transfection: suitable
• Solubilité: water: soluble
• Fluorescence: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex)
• Adéquation: suitable for nucleic acid staining
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Chaîne SMILES: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• Clé InChI: FPNZBYLXNYPRLR-UHFFFAOYSA-N

Description

Description générale

DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.

Application

Le DAPI est nettement plus sensible que le bromure d′éthidium pour marquer l′ADN dans les gels d′agarose. Il peut être utilisé dans le photofootprinting de l′ADN, pour détecter les sondes hybridées dans les applications sur membranes en permettant la visualisation spécifique des complexes double-brins, et pour évaluer les changements de l′ADN et de son contenu lors de l′apoptose par cytométrie en flux. La coloration par DAPI est également une méthode sensible et spécifique de détection des mycoplasmes.

DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).

Actions biochimiques/physiologiques

Sonde fluorescente pour l′ADN qui se lie dans le petit sillon, capable de pénétrer dans les cellules. Se lie au petit sillon d′ADN double brin (préférentiellement à l′ADN riche en AT), formant un complexe stable dont l′intensité de fluorescence est environ 20 fois plus élevée que celle du DAPI non lié.

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caractéristiques et avantages

• Ready-to-use liquid, cell permeable dye.
• Can be used with live cells and fixed cells.
• Effective for fixed-cell staining and quantitation of DNA content.
• Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.

Forme physique

DAPI readymade solution is provided at 1mg/mL concentration in water

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable