MAK521

Fumarase Assay Kit

sufficient for 100 colorimetric tests

• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Niveau de qualité: 100
• Méthode de détection: colorimetric
• Maladie(s) pertinente(s): neurological disorders; neonatal diseases
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Fumarase (or fumarate hydratase) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc.

Caractéristiques et avantages

• Linear detection range: 0.4 to 70 U/L
• Compatible with high-throughput handling systems.

Adéquation

The kit is suitable for fumarase activity determination in biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media.)

Principe

The Fumarase Assay Kit is a non-radioactive, colorimetric assay based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Informations sur la sécurité

• Pictogrammes: GHS02,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H225,H290,H315,H319,H336
• Conseils de prudence: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Central nervous system
• Code de la classe de stockage: 3 - Flammable liquids
• Classe de danger pour l'eau (WGK): WGK 3