MAK450

Glucose Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

• Synonyme(s) : GDH Activity Assay Kit
• Code UNSPSC : 12161503
• Nomenclature NACRES : NA.84

Propriétés

• Niveau de qualité: 100
• Conditions de stockage: (No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)
• Entrée: tissue; cell culture medium; tissue; plasma
• Méthode de détection: colorimetric
• Maladie(s) pertinente(s): endocrinological disorders, diabetes; hematological disorder
• Température de stockage: −20°C

Description

Description générale

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

Application

The Glucose Dehydrogenase Assay Kit may be used for:

• Metabolism Research
• Drug Discovery

Caractéristiques et avantages

• Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
• Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Adéquation

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

Principe

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅ nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations sur la sécurité

• Pictogrammes: GHS02,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H225,H290,H315,H319,H336
• Conseils de prudence: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 3 - Flammable liquids