M6444

Anti-Mitofusin 2 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-CMT2A, Anti-CMT2A2, Anti-CPRP1, Anti-KIAA0214, Anti-MARF, Anti-Mfn2
• Numéro MDL: MFCD03455396
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~86 kDa
• Espèces réactives: human, mouse, rat
• Concentration: ~1 mg/mL
• Technique(s): immunoprecipitation (IP): 5-10 μg using HeLa human epitheloid carcinoma cell lysate; western blot (chemiluminescent): 0.5-1 μg/mL using extract of mouse and rat brain mitochondria
• Numéro d'accès UniProt: O95140
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MFN2(9927) ; mouse ... Mfn2(170731) ; rat ... Mfn2(64476)

Description

Description générale

Anti-Mitofusin 2 is produced in rabbit using as immunogen a synthetic peptide corresponding to human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. Mitofusins (Mfn1 and Mfn2) form homotypic and heterotypic complexes for fusion in mitochondria.

Mitofusin-2 (MFN2) is an outer mitochondrial membrane protein.

Immunogène

synthetic peptide corresponding to amino acid residues 557-576 of human mitofusin 2 with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one and two amino acids in rat and mouse mitofusin 2, respectively.

Application

Anti-Mitofusin 2 antibody has been used in immunoblotting. It may also be used in immunoprecipitation.

Anti-Mitofusin 2 antibody produced in rabbit has been used for Western blotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

MFN2 is essential for the maintenance of mitochondrial network and controls mitochondrial metabolism. Mfn2-dependent regulatory mechanism is disturbed in obesity by reduced Mfn2 expression. Mutations in MFN2 cause Charcot Marie-Tooth neuropathy type 2A, a neurological disorder that results from degeneration of axons in peripheral nerves.

Mitofusin-2 (MFN2) plays an important role as a mitochondrial fusion protein and participates in many cell processes. It is mainly involved in regulating metabolic processes in the mitochondria. MFN2 prevents the formation of reactive oxygen species and controls endoplasmic reticulum stress. Mutations in the gene encoding this protein have been linked to diabetes and obesity.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable