M5546

Anticorps anti-c-Myc

clone 9E10, ascites fluid

• Synonyme(s) : Anti-c-Myc
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 9E10, monoclonal
• Contient: 15 mM sodium azide
• Espèces réactives: human
• Technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:100 using c-myc tagged fusion protein
• Numéro d'accès UniProt: P01106
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MYC(4609)

Description

Description générale

MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. c-myc is a member of MYC gene family. It is located on human chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.

c-Myc is a transcription factor which is over-expressed in a wide range of cancers.

Spécificité

Recognizes both N- terminal and C-terminal c-Myc tagged proteins.

Immunogène

peptide synthétique de la protéine p62^c-Myc humaine.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.

• Chromatin immunoprecipitation (1 paper)
• Enzyme-linked immunosorbent assay (1 paper)
• Immunoprecipitation (5 papers)
• Western Blotting (5 papers)
• Western blotting following immunoprecipitation(1 paper)

Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry.
Monoclonal Anti-c-Myc antibody has also been used in:

• the detection of horseradish peroxidase conjugate in phosphate buffered saline tween-20 (PBST) and 65-kDa mannoprotein (MP65) Dabs
• immunoblotting
• immunoprecipitation
• biochemical and histological studies

Actions biochimiques/physiologiques

The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as a transcriptional regulator of gene expression. MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in the poor outcome and invasive treatment, when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).

c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc.

Forme physique

The product is provided as ascites fluid with 15 mM sodium azide as a preservative.

Stockage et stabilité

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable