M0268
Milieu essentiel minimum d′Eagle
With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonyme(s) :
MEM
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About This Item
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75
Produits recommandés
Niveau de qualité
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Composants
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.
Application
Minimum Essential Medium Eagle has been used:
- in the isolation and culture of primary hepatocytes
- to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
- to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells
Caractéristiques et avantages
- Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
- The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.
Quantité
Formulated to contain 9.5 grams of powder per liter of medium.
Reconstitution
Supplement with 2.2 g/L sodium bicarbonate.
Autres remarques
Essentially the same as the original formula, except it is provided as a powder.
Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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