L6011
Lipopolysaccharides from Salmonella enterica serotype enteritidis
purified by phenol extraction
Synonyme(s) :
LPS
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About This Item
Produits recommandés
Source biologique
Salmonella enterica (Serotype enteritidis)
Niveau de qualité
Forme
lyophilized powder
Produit purifié par
phenol extraction
Impuretés
<3% Protein (Lowry)
Couleur
white to faint yellow
Solubilité
water: 0.90-1.10 mg/mL, slightly hazy to turbid, colorless to faintly yellow
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
This product is phenol extracted from Salmonella enteritidis. The source strain is ATCC 13076. Lipopolysaccharide from Salmonella enteritidis is mutagenic in murine spleen cells and causes a plaque-forming response in those cells.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4).
Actions biochimiques/physiologiques
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Notes préparatoires
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Autres remarques
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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Les clients ont également consulté
G L Cooper et al.
Infection and immunity, 62(11), 4747-4754 (1994-11-01)
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Proteinase-activated receptor-2 (PAR2) is widely expressed in the CNS but whether it plays a key role in inflammation-related behavioural changes remains unknown. Hence, in the present study we have examined whether PAR2 contributes to behaviour associated with systemic inflammation using
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L Alexopoulou et al.
The Journal of experimental medicine, 188(4), 745-754 (1998-08-26)
Lymphotoxin (LT)alpha knockout mice, as well as double LTalpha/tumor necrosis factor (TNF) knockout mice, show a severe splenic disorganization with nonsegregating T/B cell zones and complete absence of primary B cell follicles, follicular dendritic cell (FDC) networks, and germinal centers.
Huanbai Xu et al.
PloS one, 6(12), e27399-e27399 (2011-12-20)
The link between inflammation and colorectal carcinoma has been acknowledged. However, the impact of bacterial lipopolysaccharide (LPS) binding to Toll-like receptor 4 (TLR4) on chemokine receptors in human colorectal carcinoma cells still remains to be elucidated. The present study shows
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