L3126

Lipase from porcine pancreas

Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)

• Synonyme(s) : Pancreatic lipase, PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Numéro CAS: 9001-62-1
• Numéro de classification (Commission des enzymes): 3.1.1.3 (BRENDA, IUBMB)
• Numéro CE : 232-619-9
• Numéro MDL: MFCD00131509
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Porcine pancreas
• Niveau de qualité: 200
• Type: Type II
• Forme: lyophilized (cake)
• Activité spécifique: ≥125 units/mg protein (using olive oil (30 min incubation)); 30-90 units/mg protein (using triacetin)
• Composition: protein, ≥20%
• Température de stockage: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Clé InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

Application

Lipase from porcine pancreas has been used:

• for the analysis of fatty acid at the 2-position of triglycerides.
• to determine the indigestible fraction of plant source that is resistant to the action of digestive enzymes.
• as a component of dissolution media to study the dissolution profiles of theophylline.

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Actions biochimiques/physiologiques

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Qualité

Crude (Steapsin). Contains amylase and protease activity.

Définition de l'unité

One unit will hydrolyze 1.0 microequivalent of fatty acid from triacetin in 1 hr at pH 7.4 at 37°C. (pH 7.7 is used with olive oil as substrate)

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)