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KMM-101NV

Sigma-Aldrich

KOD One™ PCR Master Mix

Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR

Synonyme(s) :

Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One™ PCR Ready Mix

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About This Item

Code UNSPSC :
41106314
Nomenclature NACRES :
NA.55

Source biologique

Escherichia coli (carrying the cloned UKOD DNA polymerase gene)

Niveau de qualité

200

Forme

liquid

Utilisation

sufficient for 25 reactions
 sufficient for 500 reactions

Caractéristiques

Fast PCR
High Fidelity PCR
dNTPs included
hotstart

Technique(s)

PCR: suitable

Entrée

crude DNA

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

KOD One™ PCR master Mix is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.

This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.

Application

  • Genomic DNA amplification
  • cDNA amplification
  • Direct PCR
  • Colony PCR
  • Amplification of NGS libraries
  • Site direct gene mutation

Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA

Caractéristiques et avantages

  • Fast: Amplifies the targets using the following very short conditions:
o =1 kb: 1 sec
o 1~ 10 kb: 5 sec/ kb
o 10 kb~: 10 sec/ kb
  • Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl2.
  • High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase
  • High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
  • Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
  • High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)

Conditionnement

KMM-101NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-101NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.

Autres remarques

For R&D use only. Not for drug, household, or other uses.

Informations légales

*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.
KOD One is a trademark of Toyobo Co., Ltd.

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Moe Yokoshi et al.
Molecular cell, 78(2), 224-235 (2020-02-29)
Formation of self-associating loop domains is a fundamental organizational feature of metazoan genomes. Here, we employed quantitative live-imaging methods to visualize impacts of higher-order chromosome topology on enhancer-promoter communication in developing Drosophila embryos. Evidence is provided that distal enhancers effectively
Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network

Contenu apparenté

KOD One™ PCR Master Mix for Ultra-Fast PCR

KOD One™ PCR Master Mix overview for ultra-fast PCR with high specificity, fidelity, and yield

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