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I4144

Sigma-Aldrich

IL-3 mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonyme(s) :

IL-3, mIL-3, Interleukin-3 from mouse

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About This Item

Numéro MDL:
MFCD00164817
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Interleukin-3 from mouse, IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Source biologique

mouse

Niveau de qualité

200

Produit recombinant

expressed in E. coli

Pureté

≥98% (SDS-PAGE)

Forme

lyophilized powder

Puissance

0.01-0.1 ng/mL ED50/EC50

Qualité

endotoxin tested

Poids mol.

15.1 kDa

Conditionnement

pkg of 10 and 50 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1.000 EU/μg

Couleur

white to yellow

Numéro d'accès UniProt

P01586

Température de stockage

−20°C

Informations sur le gène

mouse ... Il3(16187)

Description générale

Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.

Application

Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.

Actions biochimiques/physiologiques

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Forme physique

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

Remarque sur l'analyse

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Characterization of phosphopeptide motifs specific for the Src homology 2 domains of signal transducer and activator of transcription 1 (STAT1) and STAT3
Wiederkehr-Adam M, et al.
Test, 278(18), 16117-16128 (2003)
Synthesis and evaluation of benzoxazole derivatives as 5-lipoxygenase inhibitors
Song H, et al.
Bioorganic & Medicinal Chemistry, 18(21), 7580-7585 (2010)
Michèle Wiederkehr-Adam et al.
The Journal of biological chemistry, 278(18), 16117-16128 (2003-02-20)
Signal transducers and activators of transcription (STAT) 1 and STAT3 are activated by overlapping but distinct sets of cytokines. STATs are recruited to the different cytokine receptors through their Src homology (SH) 2 domains that make highly specific interactions with
Interleukin-3-deficient mice have increased resistance to blood-stage malaria.
Auclair SR et al
Infection and Immunity, 82(3), 1308-1314 (2014)
Nadja Bakocevic et al.
European journal of immunology, 44(6), 1823-1834 (2014-03-13)
Basophils, a rare leukocyte population in peripheral circulation, are conventionally identified as CD45(int) CD49b(+) FcεRI(+) cells. Here, we show that basophils from blood and several organs of naïve wild-type mice express CD41, the α subunit of α(IIb)β₃ integrin. CD41 expression
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Articles

Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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