I2911

Milieu de Dulbecco modifié par Iscove

With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonyme(s) : IMDM
• Numéro MDL: MFCD00217469
• Code UNSPSC : 12352207

Propriétés

• Niveau de qualité: 400
• Stérilité: sterile-filtered
• Forme: liquid
• Technique(s): cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• Impuretés: Endotoxin, tested
• Composants: NaHCO₃: yes; glucose: yes; stable glutamine: yes; phenol red: yes
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Informations légales

StableCell is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable