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I2771

Sigma-Aldrich

ITS+3 Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonyme(s) :

ITS Supplement

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About This Item

Code UNSPSC :
12352205
Nomenclature NACRES :
NA.75

Stérilité

sterile-filtered

Niveau de qualité

300

Gamme de produits

BioReagent

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

ITS+3 liquid media supplement is a mixture of insulin, human transferrin (partially iron-saturated), sodium selenite, linoleic acid, and bovine serum albumin (BSA). It is suitable for serum-free cell culture applications.

Application

ITS+3 Liquid Media Supplement (100×) has been used as a media supplement:
  • to support the growth of human adipose stem cells (ASC)
  • to culture porcine intervertebral disc (IVD) cells and tissues
  • to culture cartilage endplate (CEP) cells

Actions biochimiques/physiologiques

  • Insulin: a polypeptide hormone that promotes glucose and amino acid uptake and may produce a mitogenic effect.
  • Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
  • Selenium: an essential trace element present in serum.

Composants

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 470 μg/ml linoleic acid, 470 μg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin.

Notes préparatoires

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Consulter la Bibliothèque de documents

Xue Liu et al.
iScience, 24(6), 102551-102551 (2021-06-22)
Pulmonary mesenchymal cells are critical players in both the mouse and human during lung development and disease states. They are increasingly recognized as highly heterogeneous, but there is no consensus on subpopulations or discriminative markers for each subtype. We completed
Di Wu et al.
International journal of stem cells, 14(1), 119-126 (2020-12-31)
The research on human hepatobiliary development and disorders has been constrained by minimal access to human fetal tissue, and low accuracy of animal models. To overcome this problem, we have established a system for the differentiation of human pluripotent stem
Hien Lau et al.
Islets, 12(3), 41-58 (2020-05-28)
The use of pancreata from pre-weaned piglets has the potential to serve as an unlimited alternative source of islets for clinical xenotransplantation. As pre-weaned porcine islets (PPIs) are immature and require prolonged culture, we developed an islet maturation media (IMM)

Articles

Linolenic Acid in Cell Culture

Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Selenium in Cell Culture

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Palmitic Acid in Cell Culture

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Palmitic Acid in Cell Culture

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

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