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Key Documents

I1784

Sigma-Aldrich

Anti-Importin α antibody, Mouse monoclonal

clone IM-75, purified from hybridoma cell culture

Synonyme(s) :

Anti-Imp α, Anti-Qip1, Anti-karyopherin α

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

IM-75, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~60 kDa

Espèces réactives

bovine, human, mouse, rat

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using HeLa cell extract

Isotype

IgG2b

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... KPNA4(3840)
mouse ... Kpna4(16649)
rat ... Kpna4(361959)

Description générale

Monoclonal Anti-Importin α (mouse IgG2b isotype) is derived from the hybridoma IM-75 produced by the fusion of mouse myeloma cells (NS1 cells), and splenocytes from BALB/c mice, immunized with recombinant human importin α. The Importin α family of proteins is comprised of three subfamilies based on amino acid sequence similarity: SRP1-like subfamily (containing the SRP1, importin α5, α6 and α7), Rch1-like subfamily (containing Rch1, pendulin, importin α2), and α3/Qip1-like subfamily (containing α4).

Immunogène

recombinant human importin α.

Application

Anti-Importin α antibody, Mouse monoclonal has been used:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunocytochemistry

Monoclonal Anti-Importin α antibody produced in mouse has been used in western blotting.

Actions biochimiques/physiologiques

The gene KPNA4 (karyopherin subunit α 4) encodes a 16kDa protein, also referred to as importin α 3 belonging to the family of karyopherins (designated KPNA1 to KPNA4). It participates in the recognition of NLS (nuclear localization sequence) sites and dock NLS-containing proteins destined for transport into the nuclear pore complex. It binds to translocon and participates in the sorting of INM (inner nuclear membrane)-directed integral membrane proteins. It associates with nucleoporins and functions in cargo-translocation across the nuclear membrane. Each KPNA contains an N-terminal repeat domain called the importin β binding site that binds to KPNB1, which in turn directs each KPNA/cargo complex through the nuclear pore. The encoded shuttling protein also functions in NF (nuclear factor)-κB nuclear translocation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Jin Xu et al.
Oncology reports, 30(4), 1878-1882 (2013-08-01)
Recent studies have shown the localization of RhoA in the cell nucleus, in addition to its cellular distribution in the cytosol and cell membrane. Our previous results that a high amount of RhoA was detected in gastric cancer cell nucleus
Guillaume Huet et al.
Journal of cell science, 126(Pt 2), 497-507 (2012-12-04)
Phactr proteins bind actin and protein phosphatase 1 (PP1), and are involved in processes ranging from angiogenesis to cell cycle regulation. Phactrs share a highly conserved RPEL domain with the myocardin-related transcription factor (MRTF) family, where actin binding to this
KPNA4 Mediates Vitamin D-Dependent Inhibition of NF-kB Activity in Swine Epicardial Preadipocytes.
Chen, et al.
Circulation, 130, A12818-A12818 (2014)
Aging impairs transcriptional regulation of vascular endothelial growth factor in human microvascular endothelial cells: implications for angiogenesis and cell survival.
Ahluwalia, A., et al.
Journal of Physiology And Pharmacology, 65 (2014)
Patrick von Morgen et al.
Biology of the cell, 110(6), 137-146 (2018-04-01)
Repair of damaged DNA is essential for maintaining genomic stability. TP53-binding protein 1 (53BP1) plays an important role in repair of the DNA double-strand breaks. Nuclear localisation of 53BP1 depends on importin β and nucleoporin 153, but the type and

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