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Key Documents

HPA008848

Sigma-Aldrich

Anti-MCAM antibody produced in rabbit

enhanced validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonyme(s) :

Anti-CD146 antigen antibody produced in rabbit, Anti-Cell surface glycoprotein MUC18 precursor antibody produced in rabbit, Anti-Cell surface glycoprotein P1H12 antibody produced in rabbit, Anti-Melanoma cell adhesion molecule antibody produced in rabbit, Anti-Melanoma-associated antigen A32 antibody produced in rabbit, Anti-Melanoma-associated antigen MUC18 antibody produced in rabbit, Anti-S-endo 1 endothelial-associated antigen antibody produced in rabbit

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Numéro HPA (Human Protein Atlas):
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Gamme de produits

Prestige Antibodies® Powered by Atlas Antibodies

Forme

buffered aqueous glycerol solution

Espèces réactives

human

Validation améliorée

orthogonal RNAseq
Learn more about Antibody Enhanced Validation

Technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:200-1:500

Séquence immunogène

LNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLE

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MCAM(4162)

Description générale

MCAM (melanoma cell adhesion molecule) is a vascular endothelium expressed cell adhesion molecule. It is ectopically expressed by malignant melanoma cells. It belongs to the immunoglobulin (Ig) superfamily. MCAM gene is a single copy gene which is localized to human chromosome 11q23, spanning 15kb and containing 16 exons. The encoded product is a transmembrane glycoprotein with a molecular weight of 113-119kDa. It is also expressed by smooth muscle cells and a subtype of activated T-cells.

Immunogène

Cell surface glycoprotein MUC18 precursor recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Actions biochimiques/physiologiques

MCAM (melanoma cell adhesion molecule) gene is linked to higher relapse probabilities in melanoma patients. It is thought to play roles in signal transduction, development, cell migration, differentiation of mesenchymal stem cells, angiogenesis and inflammatory responses. In mice, this gene is linked with tumor thickness and metastatic capabilities of human melanoma cells. The expression of this gene has potential as a prognostic marker, where the expression could correlate to poor or good outcome in melanoma patients. In late-stage osteoarthritis, this protein has potential as a marker for cartilage progenitor cell population. In gastric cancer (GC) patients, the expression of this gene is linked with lymph node metastasis and poor prognosis. Its expression is also linked to the loss of E-cadherin, and epithelial marker, and the gain of mesenchymal markers nuclear β-catenin and vimentin. Thus, in GC, this gene might be linked with epithelial-mesenchymal transition (EMT) and tumor progression.

Caractéristiques et avantages

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Liaison

Corresponding Antigen APREST71658

Forme physique

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Informations légales

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Henry J M Ferguson et al.
Oncotarget, 7(15), 20440-20454 (2016-03-05)
Current vascular-targeted therapies in colorectal cancer (CRC) have shown limited benefit. The lack of novel, specific treatment in CRC has been hampered by a dearth of specific endothelial markers. Microarray comparison of endothelial gene expression in patient-matched CRC and normal
Yi-Ning Qian et al.
CNS neuroscience & therapeutics, 20(5), 438-445 (2014-03-13)
Intraplaque neovascularization and foam cell infiltration contribute to the development of unstable plaque, leading to thromboembolism and stroke. Cell adhesion molecules (CAMs) have been reported to be involved in the progression of atherosclerosis and plaque vulnerability. The aim of this
Wen-Fang Liu et al.
International journal of molecular sciences, 13(5), 6399-6406 (2012-07-04)
CD146 has been regarded as a novel potential therapeutic target for multiple cancers. The aim of the study was to investigate the expression of CD146 in gastric cancer and evaluate its clinical-pathological and prognostic significance. The expression of CD146 and
Joseph W Wragg et al.
Cancer research, 76(8), 2314-2326 (2016-02-28)
The structure and molecular signature of tumor-associated vasculature are distinct from those of the host tissue, offering an opportunity to selectively target the tumor blood vessels. To identify tumor-specific endothelial markers, we performed a microarray on tumor-associated and nonmalignant endothelium
Christopher J Hanley et al.
Nature communications, 14(1), 387-387 (2023-02-01)
Fibroblasts are poorly characterised cells that variably impact tumour progression. Here, we use single cell RNA-sequencing, multiplexed immunohistochemistry and digital cytometry (CIBERSORTx) to identify and characterise three major fibroblast subpopulations in human non-small cell lung cancer: adventitial, alveolar and myofibroblasts.

Articles

Mesenchymal stem cell markers and antibodies suitable for investigating targets in fibroblasts, chondrocytes, adipocytes, osteoblasts, and muscle cells.

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