H9286

Anti-acetyl-Histone H3 (Ac-Lys⁹) antibody produced in rabbit

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-H3K9ac
• Numéro MDL: MFCD02685921
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~17 kDa
• Espèces réactives: bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken
• Concentration: 2-2.5 mg/mL
• Technique(s): indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate; microarray: suitable; western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate
• Numéro d'accès UniProt: P68431
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: acetylation (Lys9)
• Informations sur le gène: human ... H3F3A(3020) , H3F3B(3021) , HIST1H3A(8350) , HIST1H3B(8358) , HIST1H3C(8352) , HIST1H3D(8351) , HIST1H3E(8353) , HIST1H3F(8968) , HIST1H3G(8355) , HIST1H3H(8357) , HIST1H3I(8354) , HIST1H3J(8356) , HIST2H3A(333932) , HIST2H3C(126961) , HIST3H3(8290) ; mouse ... H3f3a(15078) , H3f3b(15081) , Hist1h3a(360198) , Hist1h3b(319150) , Hist1h3c(319148) , Hist1h3d(319149) , Hist1h3e(319151) , Hist1h3f(260423) , Hist1h3g(97908) , Hist1h3h(319152) , Hist1h3i(319153) , Hist2h3b(319154) , Hist2h3c1(15077) , Hist2h3c2(97114) ; rat ... H3f3b(117056)

Description

Description générale

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:

• indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
• microarray
• western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

Immunogène

synthetic acetylated [Ac-Lys⁹] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3.

Application

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:

• indirect immunofluorescence
• immunoblotting
• immunohistochemistry

Actions biochimiques/physiologiques

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.

In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable