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H7016

Sigma-Aldrich

Hirudin from leeches

lyophilized powder, ≥1,500 ATU/mg protein (ATU = antithrombin units)

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About This Item

Numéro CAS:
8001-27-2
Numéro CE :
232-279-1
Numéro MDL:
MFCD00081692
Code UNSPSC :
12352202

Source biologique

animal (Hirudo medicinalis)

Niveau de qualité

100
200

Forme

lyophilized powder

Activité spécifique

≥1,500 ATU/mg protein (ATU = antithrombin units)

Poids mol.

7 kDa

Solubilité

pyridine: soluble
water: soluble

Température de stockage

2-8°C

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Description générale

Hirudin is a 7 kDa acidic protein containing 65 amino acid residues that has an isoelectric point of 3.5-4.0. It is not glycosylated and lacks tryptophan, arginine and methionine residues. It is chemically stable between pH 2-12 and at temperatures up to 80°C.

Actions biochimiques/physiologiques

The anticoagulant, hirudin, is the most potent natural inhibitor of both soluble and clot-bound thrombin. It forms a high-affinity 1:1 complex with thrombin, occluding both the proteolytic site and exosite I (fibrinogen and PAR recognition site). Hirudin blocks thrombus growth and platelet activation, and has been suggested as the clinically preferred anticoagulant (over heparin and citrate) for its specific mode of action and absence of side effects. It is not metabolized in the bloodstream of humans and is eliminated unchanged via kidney filtration.

Définition de l'unité

One unit (ATU) will neutralize one NIH unit of thrombin at 37 °C, based on direct comparison to an NIH thrombin reference standard.

Remarque sur l'analyse

Protein determined by Lowry.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Sarah E Sartain et al.
Journal of immunology (Baltimore, Md. : 1950), 196(2), 832-845 (2015-12-18)
Atypical hemolytic uremic syndrome (aHUS) is a thrombotic microangiopathy with severe renal injury secondary to an overactive alternative complement pathway (AP). aHUS episodes are often initiated or recur during inflammation. We investigated gene expression of the surface complement regulatory proteins
Mengzhou Xue et al.
Brain : a journal of neurology, 132(Pt 1), 26-36 (2008-09-06)
Proteases such as matrix metalloproteinases (MMPs) and thrombin are implicated in intracerebral haemorrhage (ICH) but their interactions amongst one another and interdependency remain to be defined. The latter is important since proteases acting through different mechanisms to inflict neurotoxicity would
Yaoming Wang et al.
Stroke, 40(5), 1864-1869 (2008-12-06)
Activated protein C (APC), a protease with anticoagulant and cytoprotective activities, protects neurons and endothelium from ischemic injury. Drotrecogin-alfa activated, a hyperanticoagulant form of human recombinant APC, is currently being studied in patients with ischemic stroke. How changes in APC

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