H6660

HIV Protease Substrate 1

≥95% (HPLC), powder

• Synonyme(s) : Arg-Glu(EDANS)-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-Lys(DABCYL)-Arg
• Numéro MDL: MFCD00283442
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• product name: HIV Protease Substrate 1,
• Pureté: ≥95% (HPLC)
• Niveau de qualité: 200
• Forme: powder
• Poids mol.: 2016
• Solubilité: DMSO: soluble
• Température de stockage: −20°C

Description

Application

HIV Protease Substrate 1 has been used:

• to study protein structure-function relationships and in the fluorescent assay
• to evaluate protease inhibitors and for in vitro detection of the HIV-1 protease activity
• and in protease kinetics to study its enzymatic degradation rates

Actions biochimiques/physiologiques

HIV Protease Substrate 1 is a synthetic peptide sequence that contains the cleavage site (Tyr-Pro) for the human immunodeficiency virus (HIV) Protease. It has two covalently modified amino acids for the detection of cleavage. One modification is the addition of the fluorophore 5-(2-aminoethylamino)-1-naphthalene sulfonate (EDANS) to the glutamic acid residue, while the other one includes the addition of the acceptor chromophore 4c-dimethylaminoazobenzene-4-carboxylate (DABCYL) to the lysine residue. The modified amino acids are on opposite sides of the cleavage site. Spatial orientation and overlap of the DABCYL absorbance with the EDANS emission permit resonance energy transfer between the two moieties. However, when the peptide is cleaved by the HIV protease, the DABCYL group is no longer proximal to the fluorophore.

Substrate for endopeptidases

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)