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GE28-9893-33

Sigma-Aldrich

HiLoad® 16/600 Superdex® 75 pg

Cytiva 28-9893-33, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Synonyme(s) :

HiLoad 16/600

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

Matériaux

bed support (polyamide)
glass column
glass fiber (reinforced)

Poids mol.

3000-70000 Da (globular proteins)

Conditionnement

pkg of 1 ea

Fabricant/nom de marque

Cytiva 28-9893-33

Paramètres

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

Volume du lit

120 mL

Colonne, L × D.I.

60 cm × 16 mm

Matrice

agarose-dextran composite
 dextran-agarose particle platform
polymeric

Groupe de la matrice active

dextran-agarose phase
dextran-agarose phase

Taille moy. des particules

34 μm

cleaning

1-14

working range

3-12

Technique de séparation

size exclusion (SEC)

Description générale

HiLoad® 16/600 Superdex® 75 prep grade are prepacked 120 mL XK columns designed for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Caractéristiques et avantages

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Adéquation

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Informations légales

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Setting Column Pressure Limits for Size Exclusion Chromatography

Pressure is generated by the flow through the chromatographic system. For optimal chromatography functionality, it is important to understand the principle of the pressure drop over the different parts of a system.

Protocoles

Performing a Separation with Superdex

Superdex from GE Healthcare are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

Purification from Unclarified Cell Lysate using HisTrap™ FF Crude

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.

Sample Preparation for Size Exclusion Chromatography

Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of the chromatographic medium.

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