GE17-5442-02
Ficoll®-Paque Premium
Cytiva 17-5442-02, pack of 6 × 100 mL
Synonyme(s) :
Ficoll density gradient medium, cell culture media
About This Item
Produits recommandés
Forme
liquid
Conditionnement
pack of 6 × 100 mL
Fabricant/nom de marque
Cytiva 17-5442-02
Couleur
Colorless to slightly yellow
Plage de pH
5.5-7.5
Description générale
Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.
Application
Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).
Caractéristiques et avantages
- Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
- Manufactured under a Quality Management System certified to ISO 13485:2003.
- Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
- Cytotoxicity grade 0.
- A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Code de la classe de stockage
12 - Non Combustible Liquids
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Articles
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.
The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
This page shows methods to remove Percoll from a sample after centrifugation.
Protocoles
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
How to use percoll to prepare a gradient
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