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Principaux documents

GE17-5168-01

Sigma-Aldrich

Mono S® 5/50 GL

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About This Item

Code UNSPSC :
41115700
Nomenclature NACRES :
SB.52

ligand

-OCH2CH(OH)CH2OCH2CH(OH)CH2SO3-

Matériaux

PEEK column

Description

Ion Exchanger Type (Methyl sulfonate strong cation)

Conditionnement

pkg of 1 mL

Paramètres

580 psi

Taille du lit

5 mm × 50 mm

Volume du lit

1 mL

D.I. de la colonne

5.7 mm

Matrice

monodisperse porous polystyrene/divinyl benzene beads
polymeric

Groupe de la matrice active

methyl sulfonate phase

Taille moy. des particules

10 μm

cleaning

1-14

working range

2-12

Technique de séparation

cation exchange

Description générale

Mono S® is a strong cation exchanger prepacked with MonoBeads in a Tricorn column. The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required. The column has 1 mL bed volume for small-scale purifications.

The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.

Caractéristiques et avantages

  • Monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q ion exchangers.
  • Compatible with AKTA design, FPLC™ System, and other high-performance LC systems.
  • MonoBeads are also available as Mono P® for chromatofocusing

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Informations légales

FPLC is a trademark of Cytiva
Mono P is a registered trademark of Cytiva
Mono S is a registered trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Articles

Performing an isolation of recombinant protein complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from GE Healthcare.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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Protocoles

MonoBeads: Purification of Milligram Quantities with Highest Resolution

This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

Column packing and preparation for Ion Exchange Chromatography & Chromatafocusing

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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