GE17-5111-01

HiTrap^® IGY Purification

Cytiva 17-5111-01, pack of 1 × 5 mL

• Code UNSPSC : 23151816
• Nomenclature NACRES : NA.56

Propriétés

• ligand: 2-mercaptopyridine
• Conditionnement: pack of 1 × 5 mL
• Fabricant/nom de marque: Cytiva 17-5111-01
• Conditions de stockage: (20% Ethanol)
• Paramètres: <20 mL/min flow rate; 42 psi
• Taille du lit: 16 mm × 25 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 16 mm
• Matrice: highly cross-linked 6% agarose
• Taille moy. des particules: 34 μm
• cleaning: 2-13
• working range: 3-11
• Température de stockage: 2-30°C

Description

Description générale

HiTrap^® IgY Purification HP is a 5 mL column prepacked with Sepharose^™ High Performance for fast and easy purification of IgY from egg yolk.

Application

HiTrap^® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Caractéristiques et avantages

• Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
• Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose^™ High Performance.
• Recovery and purity of IgY are excellent.
• Can be operated with a liquid chromatography system such as ÄKTA^® design.
• The top and bottom frits are manufactured from porous polyethylene
• Connectors for usage with different chromatography systems and other equipment are included

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Autres remarques

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Informations légales

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Informations sur la sécurité

• Pictogrammes: GHS02
• Mention d'avertissement: Warning
• Mentions de danger: H226
• Conseils de prudence: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Code de la classe de stockage: 3 - Flammable liquids