Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Key Documents

GE17-0973-05

Phenyl Sepharose 6 Fast Flow (High Sub)

Cytiva 17-0973-05, pack of 200 mL

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

phenyl

Conditionnement

pack of 200 mL

Fabricant/nom de marque

Cytiva 17-0973-05

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14

working range

3-13

Adéquation

suitable for bioprocess medium

Description générale

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) medium for capture and intermediate purification requiring low to medium hydrophobicity.

Phenyl Sepharose 6 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
Phenyl Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages. Two levels of ligand substitution degree help to find the optimal selectivity and binding capacity for a given application.

Phenyl Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Phenyl Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • Standard aromatic HIC media based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages.
  • Designed for initial and intermediate purification requiring low to medium hydrophobicity
  • Two levels of substitution help find optimal selectivity and binding capacity for a given application
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

This section is about salt selection and buffer preparation in HIC medium from GE Healthcare, parameters such as buffer ions and pH are considered

The correct choice of salt and salt concentration are the most important parameters that influence capacity and final selectivity. The objective is to optimize conditions to achieve the required selectivity to bind the target protein(s) and ensure that the majority of impurities pass through the column.

This page discusses column packing and preparation techniques for reverse phase chromatography

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Afficher tout

Protocoles

Selectivity of HIC medium from GE Healthcare is based on properties such as hydrophobicity, porosity and particle size.

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

This page discusses various aspects of sample preparation for chromatographic purification.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique