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F8799

Sigma-Aldrich

Fas/Fc Chimera from mouse

>97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

Apo-1, CD95

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About This Item

Numéro MDL:
MFCD01867037
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Produit recombinant

expressed in baculovirus infected Sf21 cells

Niveau de qualité

200

Pureté

>97% (SDS-PAGE)

Forme

lyophilized powder

Puissance

0.03-0.1 μg per mL ED50 (in the presence of 20 ng/mL recombinant human Fas ligand for cytotoxicty assay of Jurkat cells)

Poids mol.

55 kDa (glycosylated form)

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Numéro d'accès UniProt

P25446

Température de stockage

−20°C

Informations sur le gène

mouse ... Fas(14102)

Description générale

Fas is a key component of apoptosis, and is a cell surface receptor. This gene is localized to human chromosome 10q24.1, and is composed of nine exons and eight introns.

Application

Fas/Fc Chimera from mouse has been used as a blocking agent during FACS (flow cytometry).

Actions biochimiques/physiologiques

Fas and its ligand play an essential role in apoptosis, by activating the cell death signaling pathway. Mutations in this gene are associated with evasion of apoptosis by cells, leading to tumorigenesis, and prevention of clearance of these cells by the immune system. A polymorphism in this gene results in the production of soluble Fas (sFas), which is implicated in the chronic autoimmune disorder called primary Sjögren′s syndrome (pSS).

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg bovine serum albumin per 1 μg of cytokine.

Remarque sur l'analyse

Biological activity was measured by its ability to inhibit Fas-ligand induced apoptosis of Jurkat cells.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Consulter la Bibliothèque de documents

Davidson D Ateh et al.
Biomaterials, 32(33), 8538-8547 (2011-08-10)
The CD95/CD95L receptor-ligand system is mainly recognised in the induction of apoptosis. However, it has also been shown that CD95L is over-expressed in many cancer types where it modulates immune-evasion and together with its receptor CD95 promotes tumour growth. Here
Cheng Jin et al.
Medical science monitor : international medical journal of experimental and clinical research, 21, 630-637 (2015-02-28)
The CD95 gene plays a key role in regulating cell growth and tumor genesis. To date, several publications have focused on the CD95 rs1800682A/G site polymorphism and various types of tumors in Asians; however, this association is still controversial and
B A Treviño-Talavera et al.
Genetics and molecular research : GMR, 13(3), 4831-4838 (2014-07-26)
Primary Sjögren's syndrome (pSS) is a chronic systemic autoimmune disease characterized by lymphocytic infiltration of exocrine glands. Soluble Fas receptor (sFas) has been suggested as a Fas-mediated apoptosis blocker that could impair clonal deletion in infiltrated autoreactive cells. The FAS
J Cheng et al.
Science (New York, N.Y.), 263(5154), 1759-1762 (1994-03-25)
Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because
A Oehm et al.
The Journal of biological chemistry, 267(15), 10709-10715 (1992-05-25)
The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the
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