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Key Documents

F8192

Sigma-Aldrich

Sérum de veau fœtal

Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonyme(s) :

FBS, FCS, sérum, sérums

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About This Item

Numéro MDL:
MFCD00132239
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Source biologique

bovine fetus

Niveau de qualité

500

Stérilité

sterile-filtered

Gamme de produits

FBS Classic

Composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

Origine

Australia origin

Technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impuretés

≤10 EU/mL endotoxin

Conditions d'expédition

dry ice

Température de stockage

−20°C

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Catégories apparentées

Description générale

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Notes préparatoires

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Remarque sur l'analyse

  • Taux d′endotoxines et d′hémoglobine testés
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Leon M Larcher et al.
Molecules (Basel, Switzerland), 24(13) (2019-07-10)
MicroRNAs (miRNAs) are short non-coding RNAs that are involved in the regulation of gene expression. Previous reports showed an over-expression of miRNA-21 (miR-21) in various cancer cells, and its up-regulation is closely related to cancer initiation, proliferation and metastasis. In
Jiamin Huang et al.
Inflammation research : official journal of the European Histamine Research Society ... [et al.], 69(3), 321-330 (2020-02-07)
To reveal the systematic response of neutrophils to sepsis and to study the hub lncRNAs in sepsis. Neutrophils taken from the femur and tibia of male C57 BL/6 mice were used in this study. And neutrophils were treated for 0 h
Marti Cabanes-Creus et al.
Molecular therapy. Methods & clinical development, 12, 71-84 (2018-12-12)
Adeno-associated virus (AAV) vectors have become one of the most widely used gene transfer tools in human gene therapy. Considerable effort is currently being focused on AAV capsid engineering strategies with the aim of developing novel variants with enhanced tropism
Hyun Bong Park et al.
Nature microbiology, 5(11), 1319-1329 (2020-07-29)
Escherichia coli is an important model organism in microbiology and a prominent member of the human microbiota1. Environmental isolates readily colonize the gastrointestinal tract of humans and other animals, and they can serve diverse probiotic, commensal and pathogenic roles in
Jianhua Chang et al.
Experimental and molecular pathology, 112, 104327-104327 (2019-11-05)
Hypoxia was proven to cause brain cell apoptosis and autophagy. Herein, we tested the influences of propofol, a commonly used intravenous sedative hypnotic drug, on apoptosis and autophagy aroused by hypoxia stimulation in PC-12 and HT-22 cells. Followed by hypoxia
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Articles

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Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.

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