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F5273

Sigma-Aldrich

Monoclonal Anti-Rat Kappa & Lambda Light Chains−FITC antibody produced in mouse

clone RT-39/RL-6, purified immunoglobulin, buffered aqueous solution

Synonyme(s) :

Monoclonal Anti-Rat Kappa & Lambda Light Chains

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Conjugué

FITC conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

secondary antibodies

Clone

RT-39/RL-6, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen 25 kDa

Conditions de stockage

protect from light

Technique(s)

dot immunobinding: 1:40
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40

Isotype

IgG1/IgG2a

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.
The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Application

Monoclonal Anti-Rat Kappa & Lambda Light Chains-FITC antibody produced in mouse has been used in:
  • fluorescent dot binding immunoassay (FDIBA)
  • immunocytochemistry
  • immunohistochemistry
  • flow cytometry

Actions biochimiques/physiologiques

FITC Monoclonal Anti-Rat kappa and lambda Light Chains may serve as an essential tool in the localization of light chains on rat immunoglobulins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

M M Aguilar et al.
The Journal of trauma, 45(1), 14-18 (1998-07-29)
T-cell response to trauma has been assessed primarily by sampling peripheral blood lymphocytes. We hypothesized that lymphocytes residing in tissue and traveling through lymph vessels are more likely to be activated by tissue injury and hemorrhage-induced hypoperfusion. We compared peripheral

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