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F4759

Sigma-Aldrich

Bovine Fibronectin

from bovine plasma, powder, suitable for cell culture

Synonyme(s) :

Fibronectin

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About This Item

Numéro CE :
289-149-2
Numéro MDL:
MFCD00131062
Code UNSPSC :
12352202
eCl@ss :
42020141
Nomenclature NACRES :
NA.75

product name

Fibronectine, powder, BioReagent, suitable for cell culture

Source biologique

bovine plasma

Niveau de qualité

200

Stérilité

sterile

Gamme de produits

BioReagent

Forme

powder

Poids mol.

~450 kDa

Conditionnement

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

Concentration

≥1 mg protein/mL

Technique(s)

cell culture | mammalian: suitable

Couverture de surface

1‑5 μg/cm2

Solubilité

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

Numéro d'accès NCBI

NM_001163778.1

Numéro d'accès UniProt

P07589

Conditions d'expédition

dry ice

Température de stockage

−20°C

Informations sur le gène

bovine ... FN1(2807940)

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Catégories apparentées

Description générale

Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.
Fibronectins are adhesive glycoproteins and are found both as cell surface proteins and in plasma. Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Application

Fibronectin from bovine plasma is used for the following applications:
  • Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
  • Cell movement assays (culture plates were coated with fibronectin)
  • Cell spreading and immunofluorescence
  • Protein specificity
  • Integration of sensor films into microfluidic chips
  • Histological and Immunohistochemical (IHC) analyses
  • Used in primary cell cultures from patients with lung carcinoma

Actions biochimiques/physiologiques

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.Study shows that human dental pulp (HDP) cells rely on fibronectin (FN) for initial attachment and subsequent spreading. FN may exhibit mitogenic activity, promoting cell proliferation, and also play a role in regulating the differentiation process of pulp cells into odontoblasts. The presence of FN appears to influence the fate and development of HDP cells, specifically in their differentiation into odontoblasts, which are responsible for dentin formation in teeth.

Attention

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

R W Redline et al.
The Journal of clinical investigation, 85(6), 1951-1958 (1990-06-01)
Because of their paternal antigens, the fetus and placenta may be considered an allograft in the maternal host. Local properties of the maternal-fetal interface, the placenta and decidua basalis, are important in preventing maternal immunologic rejection of the fetoplacental allograft.
Cigdem Aydin et al.
Human gene therapy, 18(1), 39-50 (2006-12-26)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells. However, studies have indicated that more than half of human tumors exhibit TRAIL resistance. Although the mechanism of TRAIL resistance is not
Toru Yoshihara et al.
PLoS genetics, 14(8), e1007545-e1007545 (2018-08-17)
It is uncertain which β4-galactosyltransferase (β4GalT; gene name, B4galt), β4GalT-5 and/or β4GalT-6, is responsible for the production of lactosylceramide (LacCer) synthase, which functions in the initial step of ganglioside biosynthesis. Here, we generated conditional B4galt5 knockout (B4galt5 cKO) mice, using
Wagner Shin Nishitani et al.
PloS one, 10(5), e0126440-e0126440 (2015-05-07)
A cell mechanical stimulation equipment, based on cell substrate deformation, and a more sensitive method for measuring adhesion of cells were developed. A probe, precisely positioned close to the cell, was capable of a vertical localized mechanical stimulation with a
Kristina Woodruff et al.
Scientific reports, 6, 23937-23937 (2016-04-01)
Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip
Afficher tous les articles scientifiques apparentés

Articles

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

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Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocoles

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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