E3763

Resorufin ethyl ether

≥98% (TLC), powder

• Synonyme(s) : 7-Ethoxy-3H-phenoxazin-3-one, Ethoxyresorufin, O⁷-Ethylresorufin
• Formule empirique (notation de Hill): C₁₄H₁₁NO₃
• Numéro CAS: 5725-91-7
• Poids moléculaire : 241.24
• Numéro Beilstein : 225973
• Numéro MDL: MFCD00037661
• Code UNSPSC : 12161501
• ID de substance PubChem : 24894527
• Nomenclature NACRES : NA.47

Propriétés

• Niveau de qualité: 200
• Pureté: ≥98% (TLC)
• Forme: powder
• Conditions de stockage: (Tightly closed. Dry)
• Technique(s): activity assay: suitable
• Couleur: orange to red
• Pf: 223-225 °C (lit.)
• Solubilité: chloroform: 9.80-10.20 mg/mL, clear, orange
• Adéquation: suitable for fluorescence
• Température de stockage: −20°C
• Chaîne SMILES: CCOc1ccc2N=C3C=CC(=O)C=C3Oc2c1.Fc4c(F)c(F)c(OC(=O)CNC(=O)OCC5c6ccccc6-c7ccccc57)c(F)c4F
• InChI: 1S/C23H14F5NO4.C14H11NO3/c24-17-18(25)20(27)22(21(28)19(17)26)33-16(30)9-29-23(31)32-10-15-13-7-3-1-5-11(13)12-6-2-4-8-14(12)15;1-2-17-10-4-6-12-14(8-10)18-13-7-9(16)3-5-11(13)15-12/h1-8,15H,9-10H2,(H,29,31);3-8H,2H2,1H3
• Clé InChI: ZOSYTBPPLWBBKM-UHFFFAOYSA-N

Description

Description générale

Resorufin ethers are used as markers or substrates for different cytochrome P (CYP) isoforms. Resorufin ethyl ether is the preferred resorufin ether to be metabolized by microsomes of 3-methylcholanthrene treated animals.

Resorufin is the reduced product of resazurin, also termed as Alamar Blu. It exhibits fluorescence at longer wavelengths and is flexible for modification in its hydroxyl group.

Research area: Cell Signaling

Application

Resorufin ethyl ether is suitable for the study of dealkylating activity of cytochrome P450 isozymes 1A1 and 1A2, where arylalkyl and alkyl isothiocyanates, and their glutathione, cysteine, and A′-acetylcysteine conjugates are used as inhibitors. It is suitable to study the ethoxyresorufin-O-deethylase (EROD) activity of cytochrome P450 activity (CYP1A1).

Substrats

Fluorimetric substrate for cytochrome P450 linked enzyme, CXPIA1/2

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)