E2760
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse
clone 29.1, ascites fluid
Synonyme(s) :
Anti-ERBB, Anti-ERBB1, Anti-ERRP, Anti-HER1, Anti-NISBD2, Anti-PIG61, Anti-mENA
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
29.1, monoclonal
Contient
15 mM sodium azide
Espèces réactives
pig, human, rat, mouse, canine
Ne doit pas réagir avec
rabbit
Technique(s)
indirect ELISA: 1:10,000 using extract of the A431 cell line
western blot: 1:3,000 using extract of the A431 cell line
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)
Description générale
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kDa, which spans the membranes of a wide range of normal and malignant epithelial cells. A close similarity has been found between the sequence of the avian erythroblastic leukemia viral oncogene homolog (v-erb-B) and the cytoplasmic and transmembrane part of the EGF-R (truncated EGF-R).
Spécificité
Localizes blood type A-related determinants on various glycoproteins and glycolipids in addition to specific EGF receptor determinants (only observed in human tissue). Binds an external carbohydrate on the receptor and does not block EGF binding.
Immunogène
human carcinoma cell line A431, which expresses high levels of EGF receptors.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
May be used to purify EGF receptor, for structural and functional studies of the receptor and for investigation of cell transformation versus oncogenesis.
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in
- enzyme linked immunosorbent assay (ELISA)
- immunoprecipitation
- immunoaffinity
- immunohistochemistry
- western blotting
Actions biochimiques/physiologiques
The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (Neu), ErbB3, and ErbB4. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is present on many cell types of epithelial and mesenchymal lineages. EGF R is capable of binding transforming growth factor-α and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
En option
Réf. du produit
Description
Tarif
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
B Assarsson et al.
Acta physiologica Scandinavica, 154(2), 177-183 (1995-06-01)
Members of the epidermal growth factor (EGF) family have many different effects on ovarian cells including a strong inhibition of estradiol-17 beta production. An increased binding of the EGF family to its receptor is seen at the time of ovulation.
Nanoscale imaging of epidermal growth factor receptor clustering effects of inhibitors
Abulrob A, et al.
The Journal of biological chemistry, 285(5), 3145-3156 (2010)
Epidermal growth factor receptor in relation to tumor development: EGFR gene and cancer
Mitsudomi T and Yatabe Y
The FEBS journal, 277(2), 301-308 (2010)
David J Javier et al.
Journal of nanophotonics, 2(1), 23506-23506 (2008-12-11)
Anisotropic metal-based nanomaterials have been proposed as potential contrast agents due to their strong surface plasmon resonance. We evaluated the contrast properties of gold, silver, and gold-silver hybrid nanorods for molecular imaging applications in three-dimensional biological samples. We used diffuse
Targeting the EGFR signaling pathway in cancer therapy
Seshacharyulu P et al
Expert Opinion on Therapeutic Targets, 16, 15-31 (2010)
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique