Accéder au contenu
MilliporeSigma
Toutes les photos(1)

Key Documents

View All Documentation

E1385

Sigma-Aldrich

Ethidium bromide solution

BioReagent, for molecular biology, 500 μg/mL in H2O

Synonyme(s) :

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Formule empirique (notation de Hill):
C21H20BrN3
Numéro CAS:
1239-45-8
Poids moléculaire :
394.31
Numéro Beilstein :
3642536
Numéro MDL:
MFCD00011724
Code UNSPSC :
41105322
ID de substance PubChem :
24894408
Nomenclature NACRES :
NA.31

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Concentration

500 μg/mL in H2O

Technique(s)

electrophoresis: suitable

Adéquation

suitable for gel electrophoresis

Chaîne SMILES 

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

Clé InChI

ZMMJGEGLRURXTF-UHFFFAOYSA-N

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

Ethidium bromide solution has been used to deplete mtDNA to investigate mitochondrial damage signals in autophagy. It has also been used to detect DNA damage using comet assay.
Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Actions biochimiques/physiologiques

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstitution

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 4

1 of 4

Agarose Type I-A, low EEO

Sigma-Aldrich

A0169

Agarose

Gel Loading Solution for NA electrophoresis, solution

Sigma-Aldrich

G7654

Gel Loading Solution

Acridine Orange solution 2% in H2O

Sigma-Aldrich

A9231

Acridine Orange solution

Uridine powder, BioReagent, suitable for cell culture

Sigma-Aldrich

U3003

Uridine

Autophagy-deficient Kupffer cells promote tumorigenesis by enhancing mtROS-NF-kappaB-IL1alphabeta-dependent inflammation and fibrosis during the preneoplastic stage of hepatocarcinogenesis
Sun K, et al.
Cancer letters, 388, 198-207 (2017)
Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment.
Stanic D
Chemico-Biological Interactions, 256, 134-141 (2016)
Ulrich Braunschweig et al.
Genome research, 24(11), 1774-1786 (2014-09-27)
Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron retention (IR) is the least well understood. In plants and unicellular eukaryotes, IR is
Patrick Seitz et al.
PLoS genetics, 10(1), e1004066-e1004066 (2014-01-07)
The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculative. Here we
Li Zhang et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 186, 39-51 (2014-12-04)
Bill Milsom has made seminal contributions to our understanding of ventilatory control in a wide range of vertebrates. Teleosts are particularly interesting, because they produce a 3rd, potentially toxic respiratory gas (ammonia) in large amounts. Fish are well known to
Afficher tous les articles scientifiques apparentés

Contenu apparenté

Ethidium Bromide

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique