DUO92103
Duolink® In Situ Red Starter Kit Mouse/Goat
Synonyme(s) :
in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit
About This Item
Produits recommandés
Gamme de produits
Duolink®
Niveau de qualité
Technique(s)
proximity ligation assay: suitable
Fluorescence
λex 594 nm; λem 624 nm (red) (Texas Red®; Zeiss Filter set 31)
Adéquation
suitable for fluorescence
Température de stockage
−20°C
Application
Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), red detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
The Duolink® In Situ Red Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Red fluorescence detection reagents are often used with Texas Red® filter.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Caractéristiques et avantages
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Informations légales
Composants de kit également disponibles séparément
- DUO92006Duolink® In Situ PLA® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)FDS
- DUO92001Duolink® In Situ PLA® Probe Anti-Mouse PLUSFDS
- DUO92008Duolink® In Situ Detection Reagents RedFDS
- DUO82049Duolink® In Situ Wash Buffers, FluorescenceFDS
- DUO82040Duolink® In Situ Mounting Medium with DAPIFDS
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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