D6944
Anti-Dopamine Transporter (N-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Anti-DAT, Anti-DAT1, Anti-SLC6A3, Anti-Solute carrier family 6, member 3
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Conjugué
unconjugated
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~50 kDa
Espèces réactives
mouse (predicted), rat, human
Validation améliorée
recombinant expression
Learn more about Antibody Enhanced Validation
Concentration
~1.0 mg/mL
Technique(s)
immunofluorescence: 5-10 μg/mL using SH-SY-5Y cells
western blot: 0.5-1 μg/mL using rat brain tissue extract
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SLC6A3(6531)
mouse ... Slc6a3(13162)
rat ... Slc6a3(24898)
Description générale
Dopamine transporter belongs to a family of biogenic amine transporters that include the norepinephrine, serotonin (SERT), and vesicular monoamine transporters. These transporters are members of a larger Na+/Cl--dependent neurotransmitter transporter family.
Solute carrier family 6 member 3 (SLC6A3) is mapped to human chromosome 5p15.33. It is also known as the dopamine transporter (DAT) and has a zinc binding site and 12 transmembrane region.
Immunogène
synthetic peptide corresponding to amino acids 42-59 of rat dopamine transporter conjugated to KLH. This sequence is highly conserved in mouse DAT (single amino acid substitution) and has lower homology (72% identity) to human DAT.
Application
Anti-Dopamine Transporter antibody produced in rabbit is suitable for western blot analysis at a working concentration of 0.5-1μg/mL using rat brain extract (cytosolic S1 fraction) and HEK-293T cells expressing human DAT1. It was used as a primary antibody in western analysis for dopamine transporter in several studies.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Immunocytochemistry (1 paper)
Immunohistochemistry (1 paper)
Western Blotting (1 paper)
Actions biochimiques/physiologiques
DAT regulates dopaminergic-neurotransmission. It is localized on presynaptic nerve terminals and mediates the active reuptake of dopamine from the synapse, thus terminating the neurotransmitter signal. It is the primary site for the action of neurotoxins, such as MPTP, and the drugs of abuse, cocaine and amphetamine. It has been implicated in a number of dopamine-related disorders, including attention deficit hyperactivity disorder (ADHD), bipolar disorder, clinical depression, and alcoholism. DAT may also have a role in selective vulnerability of nigrostriatal dopaminergic neurons in Parkinson′s disease as it can transport dopamine neurotoxins.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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Les clients ont également consulté
Symmetrical dimer of the human dopamine transporter revealed by cross-linking Cys-306 at the extracellular end of the sixth transmembrane segment.
Hastrup H, et al.
Proceedings of the National Academy of Sciences of the USA, 98(18), 10055-10060 (2001)
Takayuki Nakagawa et al.
PloS one, 6(9), e24865-e24865 (2011-10-08)
Repeated intermittent exposure to psychostimulants and morphine leads to progressive augmentation of its locomotor activating effects in rodents. Accumulating evidence suggests the critical involvement of the mesocorticolimbic dopaminergic neurons, which project from the ventral tegmental area to the nucleus accumbens
Enrique Garea-Rodríguez et al.
PloS one, 11(2), e0149776-e0149776 (2016-02-24)
Cerebral dopamine neurotrophic factor (CDNF) belongs to a newly discovered family of evolutionarily conserved neurotrophic factors. We demonstrate for the first time a therapeutic effect of CDNF in a unilateral 6-hydroxydopamine (6-OHDA) lesion model of Parkinson's disease in marmoset monkeys.
Mattia Volta et al.
eLife, 6 (2017-09-21)
LRRK2 mutations produce end-stage Parkinson's disease (PD) with reduced nigrostriatal dopamine, whereas, asymptomatic carriers have increased dopamine turnover and altered brain connectivity. LRRK2 pathophysiology remains unclear, but reduced dopamine and mitochondrial abnormalities occur in aged G2019S mutant knock-in (GKI) mice.
A Storch et al.
Journal of neural transmission (Vienna, Austria : 1996), 111(10-11), 1267-1286 (2004-10-14)
The carrier molecule that transports dopamine (DA) into dopamine neurons by an electrogenic, Na(+)- and Cl(-)-transport-coupled mechanism is known as the dopamine transporter (DAT). This uptake system is exclusively expressed in DA neurons with significantly higher levels of DAT expression
Articles
Drug Transport
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