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D5751

Sigma-Aldrich

Blue Dextran

Void volume marker for gel filtration columns.

Synonyme(s) :

Dextran blue from Leuconostoc mesenteroides

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About This Item

Numéro CAS:
87915-38-6
Numéro MDL:
MFCD00130661
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.25

Source biologique

non-animal source

Niveau de qualité

200

Forme

powder

Qualité

200

Poids mol.

2,000,000

Technique(s)

affinity chromatography: suitable

Adéquation

suitable for GFC marker

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Description générale

Blue Dextran is a soluble derivative of dextran. Its structure includes triazinyl dye Cibacron blue F3GA covalently bound to high molecular weight (2000 kDa) dextran. Blue Dextran is generally used as a marker to determine void volume in gel filtration chromatography columns.

Application

Blue Dextran has been used for calibration of size exclusion chromatography (SEC) column. It has also been used in Dextran blue permeability assays to determine the integrity of co-cultures containing epithelial cells. Blue dextran may be used in affinity chromatography, gel filtration chromatography, protein chromatography, and molecular weight markers. Blue dextran is also suitable to study Alzheimer′s disease.

Notes préparatoires

Prepared from dextran with an average mol. wt. of 2,000,000.

Remarque sur l'analyse

degree of substitution: 0.10 to 0.12 mmol Cibracon Blue per gram of dextran

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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USP

USP

1179800

Dextran Vo Marker

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