D4764
Deoxyribonucleic acid from calf thymus
Genomic, unsheared
Synonyme(s) :
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
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About This Item
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
41106310
eCl@ss :
32160414
Nomenclature NACRES :
NA.52
Produits recommandés
Qualité
for molecular biology
Niveau de qualité
Forme
lyophilized powder
Poids mol.
>20 kb
Température de stockage
2-8°C
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Description générale
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Spécificité
42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Application
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Caractéristiques et avantages
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Notes préparatoires
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
Reconstitution
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves
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