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D4168

Sigma-Aldrich

SIGMAFAST™ 3,3′-Diaminobenzidine tablets

tablet, To prepare 1 mL

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About This Item

Numéro CAS:
91-95-2
Numéro MDL:
MFCD00007725
Code UNSPSC :
12352204
ID de substance PubChem :
24893827
Nomenclature NACRES :
NA.83

Niveau de qualité

200

Forme

tablet

Solubilité

deionized water: 1 mL, clear, pink

Température de stockage

−20°C

Chaîne SMILES 

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

Clé InChI

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Catégories apparentées

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 1 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Informations légales

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Mention d'avertissement

Danger

Classification des risques

Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Central nervous system

Code de la classe de stockage

5.1B - Oxidizing hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

53.6 °F - closed cup

Point d'éclair (°C)

12 °C - closed cup

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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B L Sanz Ressel et al.
Journal of comparative pathology, 174, 26-33 (2020-01-21)
The molecular mechanisms contributing to the development of cutaneous papillomas (CPs) and cutaneous squamous cell carcinomas (CSCCs) are still poorly understood, limiting the ability to identify molecular suitable targets for the development of novel therapies. Persistent activation of the phosphatidylinositol
Soner Celik et al.
Journal of assisted reproduction and genetics, 35(4), 615-626 (2018-03-03)
Even with 86 live births reported globally so far, the mechanism of primordial follicle loss following autotransplantation of the frozen-thawed ovarian tissue needs further evaluation. Pten, Tsc1, p27, and Amh are the inhibitor proteins that play crucial roles in suppressing
Kristin M Byers et al.
ACS omega, 5(9), 4673-4681 (2020-03-17)
Two-dimensional paper networks (2DPNs) have enabled the use of paper-based platforms to perform multistep immunoassays for detection of pathogenic diseases at the point-of-care. To date, however, detection has required the user to provide multiple signal enhancement solutions and been limited
D Randazzo et al.
American journal of physiology. Cell physiology, 312(1), C16-C28 (2016-10-28)
We recently reported that skeletal muscle fibers of obscurin knockout (KO) mice present altered distribution of ankyrin B (ankB), disorganization of the subsarcolemmal microtubules, and reduced localization of dystrophin at costameres. In addition, these mice have impaired running endurance and
Fatma Uysal et al.
Reproduction, fertility, and development (2019-04-30)
DNA methylation plays key roles in epigenetic regulation during mammalian spermatogenesis. DNA methyltransferases (DNMTs) function in de novo and maintenance methylation processes by adding a methyl group to the fifth carbon atom of the cytosine residues within cytosine-phosphate-guanine (CpG) and
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Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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