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Key Documents

CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

Synonyme(s) :

BL21 strain

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About This Item

Code UNSPSC :
41106202
Nomenclature NACRES :
NA.85

product name

BL21(DE3) Electrocompetent Cells, for protein expression

Source biologique

Escherichia coli

Qualité

for molecular biology

Mode de croissance

adherent or suspension

Morphologie

rod shaped

Technique(s)

microbiological culture: suitable

Transformation cellulaire

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Caractéristiques et avantages

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Composants

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Code de la classe de stockage

10 - Combustible liquids

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Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows

Protocoles

BL21(DE3) Electrocompetent Cells

BL21(DE3) Electrocompetent Cells are provided in 25 μL aliquots, sufficient for one reaction. Transformation is carried out in a 0.1 cm gap cuvette. Optimal settings for electroporation are listed in the table below. Note that alternate settings result in transformation efficienes about 20-50% lower. Typical time constants are 3.5 to 4.5 msec.

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