A373-C6

97011321, human skin, Epithelial-like

• Code UNSPSC : 41106514

Propriétés

• product name: A373-C6, 97011321
• Source biologique: human skin
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial-like
• Produits: Not specified
• Récepteurs: IL-1 receptor
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human malignant melanoma IL-1 sensitive

Description de la lignée cellulaire

The cell line A375-C6 has been shown to be sensitive to the IL-1 mediated anti-proliferative effect. It has been derived from the parental line A375 (Sigma Catalogue number. 88113005) by limiting dilution together with its twin clone A375-C5 (Sigma Catalogue number. 97011320) which is IL-1 unresponsive. A375-C6 could be useful for a growth inhibition assay for IL-1. The cells have been eradicated from mycoplasma at ECACC.

Application

IL-1 growth inhibition assay, IL-1 receptor analysis

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 8
TPOX: 8,10
vWA: 16,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 1-3 x 10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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