OE21 Cell Line human

96062201, human esophagus, Epithelial

• Synonyme(s) : JROECL 21 Cells, OE 21 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: OE21 Cell Line human, 96062201, Caucasian oesophageal squamous cell carcinoma
• Source biologique: human esophagus
• Mode de croissance: Adherent
• Caryotype: Aneuploid
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: MHC class I
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human Caucasian oesophageal squamous cell carcinoma

Description de la lignée cellulaire

OE21, also known as JROECL21, was established in 1993 from a squamous carcinoma of mid oesophagus of a 74 year-old male patient. The tumour was identified as pathological stage IIA(UICC) and showed moderate differentiation. HLA-A,-B and -C (MHC class I) are expressed constitutively, expression of ICAM-1 can be induced by treatment with interferon-gamma. OE21 cells express epithelial cytokeratins and are tumourigenic in nude mice.

Application

Study of oesophageal cancer

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 12,13
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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