PNT2 Cell Line human

Normal prostate epithelium immortalized with SV40., 95012613

• Synonyme(s) : PNT-2 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human prostate
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human prostate normal, immortalised with SV40

Description de la lignée cellulaire

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.

Application

Biology of prostate cells, model of differentiated prostate epithelial cells.

PNT2 cell line has been used:

• to assess the effects of Verapamil, an L-type calcium channel blocker on autophagy
• to assess the cytotoxicity of aqueous dilutions of a universal self-priming dental adhesive (DA)
• to study the expression of soluble adenylyl cyclase (sAC) in the cells

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 6,9.3
TPOX: 8,9
vWA: 17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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