HF1-5

94101909, rat liver, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: HF1-5, 94101909
• Source biologique: rat liver
• Mode de croissance: Adherent
• Caryotype: 100 chromosomes
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat hepatoma Hybrid [H5xFao]

Description de la lignée cellulaire

HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.

Application

Gene analysis and liver function expression.

Milieu de culture

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.

Autres remarques

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.