G

94101453, rat prostate, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: G, 94101453
• Source biologique: rat prostate
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Androgen sensitive
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Dunning R-3327,prostate adenocarcinoma

Description de la lignée cellulaire

Derived from a Dunning R-3327 rat with prostatic adenocarcinoma. G is one of a group of variants which developed by a single in vivo passage. It is androgen responsive, moderately fast growing and poorly differentiated. Using this system of rat prostatic adenocarcinomas as a model, it has been demonstrated that prostatic cancer cells can become unstable at any time resulting in the addition of cytogenetically and phenotypically novel clones of cancer cells to the cancer population.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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