KYSE-70

94072012, human esophagus, Epithelial

• Synonyme(s) : KY70 Cells, KYSE70 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: KYSE-70, 94072012
• Source biologique: human esophagus
• Mode de croissance: Adherent
• Caryotype: Hypotriploid with 8% polyploidy
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human oesophageal squamous cell carcinoma

Description de la lignée cellulaire

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

Autres remarques

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