AR42J

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93100618, rat pancreas, Pancreas cells

• Code UNSPSC : 41106514

Propriétés

• Source biologique: rat pancreas
• Conditionnement: tube of 5 μg 93100618-DNA-5UG; pkg of vial of cells 93100618-1VL
• Mode de croissance: Semi-adherent aggregates
• Caryotype: Not specified
• Morphologie: Pancreas cells
• Produits: Amylase and exocrine enzymes
• Récepteurs: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat exocrine pancreatic tumour

Description de la lignée cellulaire

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

Application

AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex

Autres remarques

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