V79-4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93010723, hamster lung, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• Source biologique: hamster lung
• Conditionnement: tube of 5 μg 93010723-DNA-5UG; pkg of vial of cells 93010723-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 22
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Hamster Chinese lung

Description de la lignée cellulaire

Derived from the lung tissue of a male Chinese hamster. The cells exhibit a high plating efficiency and a doubling time of 12-14 hours. Originally used in the discovery of X-ray induced damage and recovery in mammalian cells. This is a clone of cell line V79 (Sigma Catalogue number. 86041102).

Application

Mutagenesis and mammalian cell genetic studies

Milieu de culture

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:6 to 1:14 i.e. seeding at 3x10,000-1x100,000 cells/cm²; 5% CO₂; 37°C using 0.25% trypsin/EDTA.

Autres remarques

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