JEG3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92120308, human placenta, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human placenta
• Conditionnement: tube of 5 μg 92120308-DNA-5UG; pkg of vial of cells 92120308-1VL
• Mode de croissance: Adherent
• Caryotype: modal no. 71
• Morphologie: Epithelial
• Produits: Human chorionic gonadotrophin (hCG), somatomammotrophin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human choriocarcinoma

Description de la lignée cellulaire

One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo .

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin, 5% CO₂; 37°C. Vacuolisation will occur at confluency.

Autres remarques

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