1306

90011887, human skin, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: 1306, 90011887
• Source biologique: human skin
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human skin fibroblast

Description de la lignée cellulaire

A HPRT- derivative of the SV40 transformed human fibroblast line GM0637. The cells were selected in 5μg/ml thioguanine and are useful for transfection experiments.

Application

Transfection studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 13
D16S539: 12,13
D5S818: 11
D7S820: 10
THO1: 7,9
TPOX: 9,11
vWA: 16,17

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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