Fao Cell Line from rat

89042701, rat liver, Hepatocyte

• Code UNSPSC : 41106514

Propriétés

• product name: Fao Cell Line from rat, 89042701, rat hepatoma
• Source biologique: rat liver
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Hepatocyte
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat hepatoma

Description de la lignée cellulaire

A differentiated cell line derived from H4-11-E-C3 (Sigma catalogue no. 85061112). A versatile tool for studying regulation of mono-oxygenase activities and analysing their role in the activation of xenobiotics e.g. carcinogens, drugs and pesticides.

Application

Differentiation and enzymatic studies

Milieu de culture

Coon′s modified Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) or kaign′s modified Ham′s F12 + 45mg/l asorbic acid +18mg/1 inositol + 10% FBS +2mM Glutamine

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂ 37°C.

Autres remarques

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