ZR-75-30

88113004, human breast, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Nom du produit: ZR-75-30, 88113004, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88113004, human breast, Epithelial
• Source biologique: human breast
• Conditionnement: tube of 5 μg 88113004-DNA-5UG; pkg of vial of cells 88113004-1VL
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human breast carcinoma

Description de la lignée cellulaire

Derived from ascitic fluid of a 47 year old woman with infiltrating ductal carcinoma. Ethnicity: Black.

Application

Tumourigenicity studies

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 10,11
D16S539: 9
D5S818: 12,13
D7S820: 11,12
THO1: 8,9
TPOX: 10,11
vWA: 18,19

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Cells produce a significant amount of cellular debris appearing as black particles. This cell line grows relatively slowly and requires passaging approximately every 5 days.

Autres remarques

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