C32

87090201, human skin, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: C32, 87090201
• Source biologique: human skin
• Mode de croissance: Adherent
• Caryotype: Modal no. 45
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human amelanotic melanoma

Description de la lignée cellulaire

C32 belongs to the C32r type cultures with the marker M2r substituting the original ring marker M2R. Tumours can be produced in nude mice after 1-2 months (1,000,000 cells injected).

Application

Tumourigenicity studies

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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