GH3

87012603, rat brain, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: GH3, 87012603
• Source biologique: rat brain
• Mode de croissance: Adherent
• Caryotype: Hyperdiploid, modal no. 60
• Morphologie: Epithelial
• Produits: Growth hormone, prolactin
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Wistar-Furth pituitary tumour

Description de la lignée cellulaire

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

Application

Hormone studies

Milieu de culture

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

Autres remarques

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