LLCMK2 (Original)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85062804, rhesus monkey kidney, Epithelial

• Code UNSPSC : 41106514

Propriétés

• Source biologique: rhesus monkey kidney
• Description: Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Conditionnement: tube of 5 μg 85062804-DNA-5UG; pkg of vial of cells 85062804-1VL
• Mode de croissance: Adherent
• Caryotype: 2n = 42
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Monkey Rhesus kidney

Description de la lignée cellulaire

Susceptible to a wide range of viruses including enterovirus, rhinovirus, myxovirus and poxvirus groups.

Application

LLCMK2 (Original) has been used to infect and maintain Trypanosoma cruzi parasite culture and as a medium for Sendai virus titer determination. It has also been used for viral culture.

Milieu de culture

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 5% FBS / FCS (F2442).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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